{"id":1802,"date":"2025-03-27T09:38:20","date_gmt":"2025-03-27T09:38:20","guid":{"rendered":"https:\/\/jjeci.net\/?p=1802"},"modified":"2025-04-13T09:56:59","modified_gmt":"2025-04-13T09:56:59","slug":"biosorption-of-lead-and-cadmium-from-aqueous-solutions-by-proteins-extracted-from-e-coli-cells","status":"publish","type":"post","link":"https:\/\/jjeci.net\/index.php\/2025\/03\/27\/biosorption-of-lead-and-cadmium-from-aqueous-solutions-by-proteins-extracted-from-e-coli-cells\/","title":{"rendered":"Biosorption of lead and cadmium from aqueous solutions by proteins extracted from E. coli cells"},"content":{"rendered":"\n<p>Authors: <strong>Fahmi A. Abu Al-Rub<\/strong><sup> <\/sup>, <strong>Qutaiba Ababneh<\/strong><strong>,&nbsp; Saed khayat<\/strong><strong>,<\/strong> <strong>and Shatha Badarneh<\/strong><strong>,<\/strong> <strong>&nbsp;<\/strong><\/p>\n\n\n\n<p>DOI:\u00a0<a href=\"https:\/\/doi.org\/10.48103\/jjeci8102025\">https:\/\/doi.org\/10.48103\/jjeci8102025<\/a><\/p>\n\n\n\n<p><a href=\"http:\/\/jjeci.net\">JORDANIAN JOURNAL OF ENGINEERING AND CHEMICAL INDUSTRIES (JJECI)<\/a><\/p>\n\n\n\n<p>Pages: 120-128<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><a href=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/jjeci24-124-.pdf\"><img loading=\"lazy\" decoding=\"async\" width=\"512\" height=\"512\" src=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/545456564.png\" alt=\"\" class=\"wp-image-1705\" style=\"width:50px\" srcset=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/545456564.png 512w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/545456564-300x300.png 300w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/545456564-150x150.png 150w\" sizes=\"auto, (max-width: 512px) 100vw, 512px\" \/><\/a><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<h2>\u00a0<\/h2>\n<h2><strong>Abstract<\/strong><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-1741\" src=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-1-225x300.jpeg\" alt=\"\" width=\"157\" height=\"210\" srcset=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-1-225x300.jpeg 225w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-1-768x1024.jpeg 768w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-1.jpeg 960w\" sizes=\"auto, (max-width: 157px) 100vw, 157px\" \/>\u00a0 <img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-1742\" src=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-2-300x225.jpeg\" alt=\"\" width=\"253\" height=\"190\" srcset=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-2-300x225.jpeg 300w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-2-1024x768.jpeg 1024w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-2-768x576.jpeg 768w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-2.jpeg 1280w\" sizes=\"auto, (max-width: 253px) 100vw, 253px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-1743\" src=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-3-225x300.jpeg\" alt=\"\" width=\"173\" height=\"231\" srcset=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-3-225x300.jpeg 225w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-3-768x1024.jpeg 768w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-3.jpeg 960w\" sizes=\"auto, (max-width: 173px) 100vw, 173px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-1744\" src=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-4-225x300.jpeg\" alt=\"\" width=\"164\" height=\"219\" srcset=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-4-225x300.jpeg 225w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-4-768x1024.jpeg 768w, https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/WhatsApp-Image-2025-03-10-at-9.27.15-PM-4.jpeg 960w\" sizes=\"auto, (max-width: 164px) 100vw, 164px\" \/><\/p>\n<p>The potential use of bacterial-based protein extracts for heavy metal removal from aqueous solution was investigated. The proteins-based biosorbent were extracted from wild-type <em>E. coli<\/em> strain MG16755 using sonication. The metal ions adsorption efficiency was determined in batch mode. The maximum metal ions adsorption capacity was achieved after 24 hours at C and pH=6. Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated that the protein extract is capable of interacting with heavy metal ions through its functional groups (O-H \/ N-H, C=O, and C-O \/ P-O). The equilibrium data aligned well with the Langmuir model, indicating that the protein extract&#8217;s maximum adsorption capacities ( ) for Pb (II) and Cd (II) were 204 mg\/g and 164 mg\/g, respectively. The Dubinin-Radushkevich (DRK) isotherm model estimated the mean free energy to be 0.7 kJ\/mol for both Pb (II) and Cd (II), indicating that the adsorption process is primarily physical in nature. Additionally, the adsorption of Pb (II) and Cd (II) followed pseudo-second-order kinetics most closely. The findings of this study provide fundamental recommendations for the optimal utilization of EPS from <em>E. coli<\/em> in bioremediation for wastewater treatment.<\/p>\n<p><strong>\u00a0<\/strong><strong>Paper type<\/strong>: Research paper<\/p>\n<p><strong>Keywords:<\/strong><em>.<\/em> Adsorption, Protein, Heavy metals, Escherichia coli.<\/p>\n<p><strong>Citation: <\/strong>Al-Rub, F., Ababneh, Q., khayat, S., \u00a0and <strong>\u00a0<\/strong>Shatha, B. .\u201c Biosorption of lead and cadmium from aqueous solutions by proteins extracted from E. coli cells\u201d, Jordanian Journal of Engineering and Chemical Industries,<\/p>\n<p>\u00a0Vol. 8, No.1, pp: 120-128 (2025).<\/p>\n\n\n<div style=\"height:67px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-pdf-viewer-block-standard\" style=\"text-align:left\"><div class=\"uploaded-pdf\"><a href=\"https:\/\/jjeci.net\/wp-content\/uploads\/2025\/03\/jjeci24-124-.pdf\" data-width=\"\" data-height=\"\"><\/a><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Authors: Fahmi A. Abu Al-Rub , Qutaiba Ababneh,&nbsp; Saed khayat, and Shatha Badarneh, &nbsp; DOI:\u00a0https:\/\/doi.org\/10.48103\/jjeci8102025 JORDANIAN JOURNAL OF ENGINEERING AND CHEMICAL INDUSTRIES (JJECI) Pages: 120-128 \u00a0 Abstract \u00a0 The potential use of bacterial-based protein extracts for heavy metal removal from aqueous solution was investigated. The proteins-based biosorbent were extracted from wild-type E. coli strain MG16755 &#8230;<\/p>\n","protected":false},"author":1,"featured_media":1692,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[65,66],"tags":[],"class_list":{"0":"post-1802","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-65","8":"category-volume-8-issue-1"},"_links":{"self":[{"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/posts\/1802","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/comments?post=1802"}],"version-history":[{"count":2,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/posts\/1802\/revisions"}],"predecessor-version":[{"id":1831,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/posts\/1802\/revisions\/1831"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/media\/1692"}],"wp:attachment":[{"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/media?parent=1802"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/categories?post=1802"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jjeci.net\/index.php\/wp-json\/wp\/v2\/tags?post=1802"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}